Format:
Online-Ressource (390 p)
ISBN:
9780409900279
Content:
Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmid
Note:
Description based upon print version of record
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Front Cover; Maximizing Gene Expression; Copyright Page; Contributors; Table of Contents; Preface; Chapter 1. E. Coli Promoters; 1.1 Defining Promoters; 1.2 Structure Analysis; 1.3 Steps in Transcription Initiation; 1.4 Structure-Function Correlation; 1.5 Regulation of Transcription Initiation; 1.6 Conclusion; References; Chapter 2. Yeast Promoters; 2.1 Transcription in Yeast; 2.2 Methods for Studying Yeast Promoters; 2.3 Upstream Promoter Elements; 2.4 The TATA Promoter Element; 2.5 Elements that Select Initiation Sites; 2.6 Transcription Regulation; 2.7 Regulatory Proteins
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2.8 Other Aspects of Regulation2.9 Complex Promoter Organization; 2.10 Molecular Mechanisms: Inferences and Speculations; References; Chapter 3. Protein Coding Genes of Higher Eukaryotes: Promoter Elements and trans-Acting Factors; 3.1 The TATA Box and the Cap Site; 3.2 The Upstream Promoter Elements; 3.3 Enhancer Elements; 3.4 Other trans-Acting Factors; 3.5 Conclusion and Prospects; References; Chapter 4. The Instability of Messenger RNA in Bacteria; 4.1 Some Fundamental Observations and Their Significance; 4.2 Mechanistic Models; 4.3 Search for Specific Enzymes for mRNA Degradation
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4.4 The Search for Targets4.5 Searching for New Ends; 4.6 Searching for a Model; 4.7 Conclusions; References; Chapter 5. Replication Control of the ColE1-Type Plasmids; 5.1 Incompatibility; 5.2 Replication of ColE1 DNA in Vitro; 5.3 RNA I and Primer Processing; 5.4 RNA I Secondary Structure; 5.5 Mutations in RNA I and Primer That Define Domains of Interaction; 5.6 Analysis of the RNA I-Primer Interaction; 5.7 The Replication Primer; 5.8 Replication-Defective Mutants; 5.9 Temperature-Sensitive Replication Mutants; 5.10 The rop Function; 5.11 Partition and Stability Functions
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5.12 Host Contributions to ColE1 Replication5.13 On the Control of the Control Elements; 5.14 Implications for Maximizing Gene Expression; References; Chapter 6. Copy Number and Stability of Yeast Plasmids; 6.1 Vectors for DNA Cloning in Saccharomyces Cerevisiae; 6.2 Elements of the 2μ Circle Replication System; 6.3 Factors Affecting the Stability and Copy Number of Hybrid2µ-Based Plasmids; 6.4 Conclusion; References; Chapter 7. Translation Initiation; 7.1 Prokaryotes; 7.2 Eukaryotes; 7.3 Conclusion; References
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Chapter 8. Biased Codon Usage: An Exploration of Its Role in Optimization of Translation8.1 Codon Usage; 8.2 Physiological Aspects of Codon Usage; 8.3 Codon Context and tRNA-tRNA Interaction; 8.4 Evolutionary Aspects of Codon Usage; 8.5 Altering Codon Bias Experimentally; References; Chapter 9. The Selective Degradation of Abnormal Proteins in Bacteria; 9.1 Proteins Rapidly Hydrolyzed in E. Coli; 9.2 Intracellular Aggregates of Abnormal Polypeptides; 9.3 The Energy Requirement and Pathway for Protein Breakdown; 9.4 ATP-Stimulated Proteolysis in Cell-Free Extracts
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9.5 Proteolytic Enzymes in E. Coli
Additional Edition:
9781483100807
Additional Edition:
Print version Maximizing Gene Expression
Language:
English
Keywords:
Electronic books
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