In:
Hypertension, Ovid Technologies (Wolters Kluwer Health), Vol. 33, No. 1 ( 1999-01), p. 373-377
Kurzfassung:
Abstract —Angiotensin (Ang) ΙΙ stimulates secretions of aldosterone and an endogenous ouabain-like steroid (EO) from bovine adrenal zona glomerulosa (BAG) cells. The BAG cell sodium pump, a possible target of EO, affects aldosterone secretion although little is known about this pump. Here, we describe the effects of Ang II on the characteristics of this transporter and steroid secretions. Under serum-free conditions, 3 H-ouabain bound to a single class of sites on BAG cells. Binding of label was time and concentration dependent, was sensitive to extracellular potassium ions, and was displaced by ouabain and digoxin with EC 50 of ≈218 and ≈232 nmol/L, respectively. Sodium pump–mediated 86 Rb uptake was inhibited by ouabain (EC 50 ≈301 nmol/L). Ang II dose dependently augmented secretions of EO and aldosterone, increased ouabain-sensitive 86 Rb uptake and 3 H-ouabain binding, and increased the affinity for 3 H-ouabain binding ( K d , from 205 to 80 nmol/L) with no change in the maximal number of sodium pumps (5.45×10 6 ) per cell. Losartan blocked all effects of Ang II except EO secretion, which was inhibited by PD123319. We conclude that BAG cells express sodium pumps in high density and bind ouabain to a single class of low-affinity sites. The characteristics of the sodium pumps protect BAG cells from EO autotoxicity but may exclude them from mediating feedback inhibition of EO secretion. The effects of Ang II on sodium pump activity, ouabain binding affinity, and aldosterone secretion are mediated via Ang II type 1 receptors, whereas Ang II type 2 receptors augment EO secretion. The role of the Ang II–mediated increase in the ouabain sensitivity of BAG cell sodium pumps in the secretions of aldosterone and EO remains to be elucidated.
Materialart:
Online-Ressource
ISSN:
0194-911X
,
1524-4563
DOI:
10.1161/01.HYP.33.1.373
Sprache:
Englisch
Verlag:
Ovid Technologies (Wolters Kluwer Health)
Publikationsdatum:
1999
ZDB Id:
2094210-2
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