Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: Lupus‐prone NZB/W F1 mice develop glomerulonephritis after T helper cell‐dependent isotype switching of autoantibody secretion from IgM to IgG at about 6 months of age. We compared innate immune natural killer (NK) T cells and conventional T cells for their capacity to help spontaneous in vitro immunoglobulin and autoantibody secretion of innate immune (B‐1 and marginal zone) and conventional (follicular) B cell subsets from NZB/W F1 mice. We found that purified NKT cells not only increased spontaneous secretion of IgM and IgM anti‐double‐stranded (ds) DNA antibodies by B‐1 and marginal zone B cells, but also facilitated secretion of IgG anti‐dsDNA antibodies predominantly by B‐1 B cells. Few IgM or IgG anti‐dsDNA antibodies were secreted by follicular B cells, and conventional T cells failed to provide potent helper activity to any B cell subset. All combinations of T and B cell subsets from normal C57BL/6 mice failed to generate vigorous IgM and IgG secretion. NZB/W NKT cell helper activity was blocked by anti‐CD1 and anti‐CD40L mAb. In conclusion, direct interactions between innate immune T and B cells form a pathway for the development of IgM and IgG lupus autoantibody secretion in NZB/W mice.
In:
volume:38
In:
number:1
In:
year:2007
In:
pages:156-165
In:
extent:10
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 38, Heft 1 (2007), 156-165 (gesamt 10), 1521-4141
Language:
English
DOI:
10.1002/eji.200737656
URN:
urn:nbn:de:101:1-2023062406101368925899
URL:
https://doi.org/10.1002/eji.200737656
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023062406101368925899
URL:
https://d-nb.info/1293758418/34
URL:
https://doi.org/10.1002/eji.200737656
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