Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: Although it is known that a single peptide can be recognized by CTL restricted to two MHC class I alleles, there is no direct evidence for presentation of a single peptide by two MHC class I molecules. Furthermore, it is unclear whether such peptides are presented to the same T cell or to different T cells. Our previous study suggested that CTL recognition of the human immunodeficiency virus‐1 (HIV‐1) Pol HIV‐B35‐SF2‐24 epitope (IPLTEEAEL) occurs via both HLA‐B35 and HLA‐B51 restriction. Here we provide the first direct evidence that a single CTL clone can recognize this peptide presented by both HLA‐B35 and HLA‐B51. Furthermore, we directly purified this peptide eluted from both HLA‐B*3501 and HLA‐B*5101 molecules isolated from target cells infected with HIV‐1 recombinant vaccinia virus. These results demonstrate that HIV‐B35‐SF2‐24 is a naturally processed peptide which is presented by both HLA‐B*3501 and HLA‐B*5101. TCR analysis of one CTL clone suggested that it is a single clone. B*3501‐SF2‐24‐tetrameric complexes inhibited both HLA‐B*3501‐ and HLA‐B*5101‐restricted recognition of this clone, suggesting that the TCR of this clone cross‐recognize the structure of both HLA class I‐peptide complexes.
In:
volume:30
In:
number:9
In:
year:2000
In:
pages:2521-2530
In:
extent:10
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 30, Heft 9 (2000), 2521-2530 (gesamt 10), 1521-4141
Language:
English
DOI:
10.1002/1521-4141(200009)30:9〈2521::AID-IMMU2521〉3.0.CO;2-Q
URN:
urn:nbn:de:101:1-2023110506413963466019
URL:
https://doi.org/10.1002/1521-4141(200009)30:9〈2521::AID-IMMU2521〉3.0.CO;2-Q
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023110506413963466019
URL:
https://d-nb.info/1308669003/34
URL:
https://doi.org/10.1002/1521-4141(200009)30:9〈2521::AID-IMMU2521〉3.0.CO;2-Q
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