Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: Stimulation of the gp130 signaling pathway by IL‐6 is known to contribute significantly to hematopoietic expansion in vitro, mostly in combination with other cytokines. In the present study we have investigated whether a similar effect can be observed also in vivo using shortterm assays in which irradiated mice were analyzed for repopulation of lymphoid organs. Mice were injected with a combination of soluble IL‐6Rα either with wild‐type (wt) human IL‐6 or with an IL‐6 variant, called K‐7/D‐6, that shows a 70‐fold higher IL‐6Rα affinity. We observed that while wt IL‐6 was able to induce a partial effect only in combination with IL‐3, K‐7/D‐6 bypassed the need for IL‐3 and yielded complete recovery. In lethally irradiated mice reconstituted with syngeneic bone marrow cells K‐7/D‐6 strongly accelerated the repopulation of thymus and spleen and hastened blood neutrophil recovery. These results underscore the potential of the gp130 signaling pathway in hematopoietic reconstitution after myeloablative regimens and open the possibility to fully exploit it with a super‐active IL‐6 variant.
In:
volume:29
In:
number:1
In:
year:2006
In:
pages:300-310
In:
extent:11
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 29, Heft 1 (2006), 300-310 (gesamt 11), 1521-4141
Language:
English
DOI:
10.1002/(SICI)1521-4141(199901)29:01〈300::AID-IMMU300〉3.0.CO;2-J
URN:
urn:nbn:de:101:1-2023122205473045472880
URL:
https://doi.org/10.1002/(SICI)1521-4141(199901)29:01〈300::AID-IMMU300〉3.0.CO;2-J
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023122205473045472880
URL:
https://d-nb.info/1314375784/34
URL:
https://doi.org/10.1002/(SICI)1521-4141(199901)29:01〈300::AID-IMMU300〉3.0.CO;2-J
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