Format:
Online-Ressource (PDF-Datei: 88 S., 2897 KB)
,
Ill., graph. Darst.
Content:
Biomaterial, Biokompatibilität, In vivo, Entzündung, Immunhistochemie, Cytokine, Implantation, Titan, Niedertemperaturplasma, Plasmapolymerisation, Allylamin, Acrylsäure
Content:
The aim of the thesis was an examination of the local and systemic inflammatory reactions following intramuscular implantation of titanium (Ti) plates modified with low-temperature plasma-polymer processes. This originated from previous cell culture experiments with Ti samples coated either with a positively charged plasma-polymerized allylamine film (PPAAm) or a negatively charged plasma-polymerized acrylic acid film (PPAAc). These in vitro studies demonstrated positive effects for a coating with PPAAm regarding the growth of osteoblasts and for a coating with PPAAc regarding the differentiation of human mesenchymal stem cells. For the in vivo evaluation performed in this thesis, it was basically necessary to establish a method for quantitative histological examination of the local tissue reactions after implantation of Ti samples. Its evaluation showed good reproducibility of the obtained results and thus the suitability of this digital image procedure for the subsequent investigations. Using this method, a morphometric immunohistological examination of the local inflammatory tissue response after intramuscular implantation of PPAAc- and PPAAm-coated Ti plates was performed. In a first study, the total monocytes and macrophages as well as MHC class II antigen-presenting cells were examined in the peri-implant tissue after 56 days for both coatings in comparison to uncoated control samples. The results demonstrated a stronger chronic inflammatory response for PPAAc-coated ...
Note:
Greifswald, Univ., Diss., 2014
Additional Edition:
Druckausg. Walschus, Uwe Differenzierte Untersuchung der lokalen und systemischen Entzündungsreaktionen nach Implantation von Niedertemperatur-Plasmapolymer-modifizierten Titanplättchen im Tiermodell Ratte 2013
Language:
German
Keywords:
Biomaterial
;
Biokompatibilität
;
In vivo
;
Entzündung
;
Immuncytochemie
;
Cytokine
;
Implantation
;
Titan
;
Kaltes Plasma
;
Plasmapolymerisation
;
Allylamin
;
Acrylsäure
;
Hochschulschrift
URN:
urn:nbn:de:gbv:9-001941-9
URL:
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