Format:
Online-Ressource
ISSN:
1439-7633
Content:
Abstract: Directed enzyme evolution has proven to be a powerful tool for improving a range of properties of enzymes through consecutive rounds of diversification and selection. However, its success depends heavily on the efficiency of the screening strategy employed. Fluorescence‐activated cell sorting (FACS) has recently emerged as a powerful tool for screening enzyme libraries due to its high sensitivity and its ability to analyze as many as 108 mutants per day. Applications of FACS screening have allowed the isolation of enzyme variants with significantly improved activities, altered substrate specificities, or even novel functions. This review discusses FACS‐based screening for enzymatic activity and its potential application for the directed evolution of enzymes, ribozymes, and catalytic antibodies.
In:
volume:10
In:
number:17
In:
year:2009
In:
pages:2704-2715
In:
extent:12
In:
ChemBioChem, Weinheim : Wiley-VCH, [2000]-, 10, Heft 17 (2009), 2704-2715 (gesamt 12), 1439-7633
Language:
English
DOI:
10.1002/cbic.200900384
URN:
urn:nbn:de:101:1-2023041607093734896112
URL:
https://doi.org/10.1002/cbic.200900384
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023041607093734896112
URL:
https://d-nb.info/1286361710/34
URL:
https://doi.org/10.1002/cbic.200900384
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