Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: Metastasis remains a significant cause to cancer‐related mortality, underscoring the critical need for early detection and analysis of circulating tumor cells (CTCs). This study presents a novel microfluidic chip designed to efficiently capture A549 lung cancer cells by combining dielectrophoresis (DEP) and aptamer‐based binding, thereby enhancing capture efficiency and specificity. The microchip features interdigitated electrodes made of indium‐tin‐oxide that generate a nonuniform electric field to manipulate CTCs. Following three chip design, scenarios were investigated: (A) bare glass surface, (B) glass modified with gold nanoparticles (AuNPs) only, and (C) glass modified with both AuNPs and aptamers. Experimental results demonstrate that AuNPs significantly enhance capture efficiency under DEP, with scenarios (B) and (C) exhibiting similar performance. Notably, scenario (C) stands out as aptamer‐functionalized surfaces resisting fluid shear forces, achieving CTCs retention even after electric field deactivation. Additionally, an innovative reverse pumping method mitigates inlet clogging, enhancing experimental efficiency. This research offers valuable insights into optimizing surface modifications and understanding key factors influencing cell capture, contributing to the development of efficient cell manipulation techniques with potential applications in cancer research and personalized treatment options.
In:
day:04
In:
month:01
In:
year:2024
In:
extent:11
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, (04.01.2024) (gesamt 11), 1522-2683
Language:
English
DOI:
10.1002/elps.202300206
URN:
urn:nbn:de:101:1-2024010514032287759290
URL:
https://doi.org/10.1002/elps.202300206
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024010514032287759290
URL:
https://d-nb.info/1315166933/34
URL:
https://doi.org/10.1002/elps.202300206
Bookmarklink