Format:
1 Online-Ressource (80 p)
ISBN:
9783319462400
Content:
Preface -- Contents -- About the Editors -- 1 Introduction to Capillary Electrophoresis-Mass Spectrometry -- 1.1 Capillary Electrophoresis -- 1.2 Capillary Electrophoresis Coupled with Mass Spectrometry (CE-MS) -- 1.3 Method Development in CE-MS for Protein Analysis -- 1.4 Advanced Application of CE-MS in Therapeutic Protein Analysis -- References -- 2 Coated Capillaries for CE-MS of Therapeutic Protein -- 2.1 Introduction to Capillary Coating -- 2.2 Types of Capillary Coating -- 2.3 Challenges in Coating and the Use of Coated Capillaries
Content:
2.4 Case Study of Coated Capillaries Used for Protein CE-MS Analysis -- 2.4.1 Analysis of Recombinant Human Erythropoietin (RHuEPO) Using Neutral and Cationic Dynamic Coating -- 2.4.2 Analysis of Recombinant Human Chorionic Gonadotropin -- 2.4.3 Analysis of Recombinant Human Growth Hormone -- 2.4.4 Analysis of Reduced Monoclonal Antibody Using LPA Capillary -- 2.4.5 Analysis of Interferon-β1 Using Cross-Linked Polyethylenimine Coating -- 2.4.6 Capillary Coating by Bovine Serum Albumin -- References
Content:
3 Therapeutic Monoclonal Antibody Intact Mass Analysis by Capillary Electrophoresis-Mass Spectrometry -- 3.1 Introduction -- 3.2 CE-MS Coupling -- 3.2.1 Interfaces -- 3.2.1.1 Sheath-Flow Interface -- 3.2.1.2 Sheathless Interface -- 3.2.1.3 EOF-Driven Sheath-Flow Nanospray Interface -- 3.2.2 Mass Spectrometer Ionization Sources -- 3.2.2.1 CE-ESI-MS -- 3.2.2.2 CE-UVMALDI -- 3.3 New Device-Chip-Based CE-MS -- 3.4 Application: Intact MAb Mass by Coupling CE to TOF-MS with an EOF Nanoflow Interface -- 3.4.1 Materials -- 3.4.1.1 Separation Capillary -- 3.4.1.2 Instrumentation
Content:
3.4.1.3 Samples and Reagents -- 3.4.1.4 Stock Solutions -- 3.4.1.5 Standard Protein Preparation -- 3.4.1.6 Deglycosylation of IgG1 -- 3.4.1.7 IgG1 Reduction -- 3.4.1.8 Ide S Enzyme Digestion -- 3.4.1.9 Sample DesaltingBuffer Exchanging -- 3.4.1.10 CE-MS Sample Preparation -- 3.4.2 Results -- 3.4.2.1 CE-MS Analysis of Protein Standards -- 3.4.2.2 Reduced Monoclonal Antibody (Heavy Chain and Light Chain) -- 3.4.2.3 Analysis of Deglycosylated IgG1 -- 3.4.2.4 Analysis of Ide S Enzyme Digested Monoclonal Antibody (Fab'2 and Fc2) and Reduced Ide S Enzyme Digested Monoclonal Antibody (Fd, LC and Fc2)
Content:
3.4.2.5 Case Study: CE-MS Analysis of Chemically Stressed IgG1 Sample -- 3.4.3 Notes -- 3.4.3.1 System Optimization -- 3.4.3.2 Background Electrolyte Composition -- 3.4.3.3 Sample Injection -- 3.4.3.4 Selection of Separation Capillary -- 3.4.3.5 Pressure for Sample Injection -- 3.4.3.6 Capillary Length and Separation Voltage -- 3.4.3.7 Sample Desalting -- 3.4.3.8 Height of Capillary Inlet and Outlet -- 3.4.3.9 Intact Mass Analysis for Recombinant Monoclonal Antibody Therapeutics -- 3.4.4 Conclusions -- References
Content:
4 Separation and Characterization of Proteins and Peptides in Biological Fluids Using CE-MS
Note:
Description based upon print version of record
Additional Edition:
9783319462387
Additional Edition:
Print version Xia, James Q Capillary Electrophoresis-Mass Spectrometry : Therapeutic Protein Characterization Cham : Springer International Publishing,c2016 9783319462387
Language:
English
Keywords:
Electronic books
URL:
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