Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: This paper describes approaches for large‐volume sample stacking (LVSS) with an EOF pumpin CE for the determination of methotrexate (MTX) and its metabolites in human plasma. After pretreatment of plasma through a SPE cartridge, a large sample volume was loaded by hydrodynamic injection (3 psi, 70 s) into the capillary filled with phosphate buffer (70 mM, pH 6.0) containing 0.01% polyethylene oxide. Following removal of a large plug of sample matrix from the capillary using polarity switching (—25 kV), the separation of anionic analytes was subsequently performed without changing polarity again, achieving an improvement of sensitivity of around a 100‐fold. The method was applied to therapeutic drug monitoring of MTX in one acute lymphoblastic leukemia patient. This study is one of very few applications showing the feasibility of LVSS in analysis of biological samples by CE.
In:
volume:27
In:
number:14
In:
year:2006
In:
pages:2905-2909
In:
extent:5
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 27, Heft 14 (2006), 2905-2909 (gesamt 5), 1522-2683
Language:
English
DOI:
10.1002/elps.200500800
URN:
urn:nbn:de:101:1-2023090905204348884839
URL:
https://doi.org/10.1002/elps.200500800
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023090905204348884839
URL:
https://d-nb.info/1301973084/34
URL:
https://doi.org/10.1002/elps.200500800