Format:
XIX, 150 Seiten
,
Diagramme, Illustrationen
Content:
The development of type 2 diabetes (T2D) is driven by genetic as well as life style factors. However, even genetically identical female NZO mice on a high-fat diet show a broad variation in T2D onset. The main objective of this study was to elucidate and investigate early epigenetic determinants of type 2 diabetes. Prior to other experiments, early fat content of the liver (〈55.2 HU) in combination with blood glucose concentrations (〉8.8 mM) were evaluated as best predictors of diabetes in NZO females. Then, DNA methylome and transcriptome were profiled to identify molecular pathophysiological changes in the liver before diabetes onset. The major finding of this thesis is that alterations in the hepatic DNA methylome precede diabetes onset. Of particular interest were 702 differentially methylated regions (DMRs), of which 506 DMRs had genic localization. These inter-individual DMRs were enriched by fivefold in the KEGG pathway type 2 diabetes mellitus, independent of the level of gene expression, demonstrating an epigenetic predisposition toward diabetes. Interestingly, among the list of hepatic DMRs, eleven DMRs were associated with known imprinted genes in the mouse genome. [...]
Note:
Dissertation Universität Potsdam 2021
Language:
English
Keywords:
Hochschulschrift
;
Dissertation
;
Hochschulschrift
Author information:
Schürmann, Annette 1960-
Author information:
Kühnen, Peter 1977-
Author information:
Püschel, Gerhard 1958-
